My Anti-Aging Protocol Broke a World Record...

Foreign [Music] Today, Oliver and I are going to go over the Blueprint Protocol. This is what we've been up to for the past 18 months or so, and we've got it structured in routine measurements. We look at different body, different organs, and then the protocols we have for each. So hopefully, the objective is to give you a high-level overview of the entirety of what we're doing. And then also, there are some specific things which hopefully are useful to you.

But a lot of people, uh, viewing this—mouse, I'm looking in—haven't seen anything like this. We're not aware of others who have gone to this extent in measurement protocol and regeneration and data production. And so, yeah, we just want to provide a 360-degree view of this, provide you some behind-the-scenes stories of stuff, and, uh, yeah, just just bring you along for the ride with us as we go. This is an open science project; we share everything—the measurements, protocols, the data—and hoping this will be helpful to the entire field, anyone who's interested in health and wellness.

Yeah, so we're trying to quantify the biological age of all 70 organ types in Brian's body and maximally reduce the age ongoing. Then, so you see here, like we basically try to build a daily protocol, uh, from the ground up, from the moment I wake up to the end of the day. We try to systematize everything, so every single calorie I ate, uh, the minute I go to bed, we try to assist. I can't look at my blood draw; it's even hard for me to talk as I'm getting stuck.

Okay, do you have that same problem?

Yeah, I, you know, even as a doctor, I can't look at my own blood being drawn; it's too traumatizing for me.

Ah, so you turn your head?

Oh, yeah, and you keep it turned, or once it's in, you look back?

I'll keep it turned, give it turned. Yeah, and then do you say, "Hey, how's it going? Did you check in?"

Oh, well, yeah, I make some small talk, or you know, some, uh, I try and describe myself as much as possible. I always ask the phlebotomist, like, "Okay, so we're doing one stick today, right? Like there's no," and there's no fishing, like, if we miss the vein, you know, like poking around.

Yeah, luckily we both have six percent body fat, so it's quite easy to draw from us, which is good.

Yeah, so we, so Oliver and I have, uh, built this protocol, and it's again, in any given day, it's, I don't know, maybe 200 things that we've implemented that all have that are based on evidence and peer-review publications. And then we try to implement it so that we never think about it ever again, um, yeah, because it's very hard what we're doing, you know, hundreds of tests per year across, uh, you know, imaging, MRI, ultrasound, blood tests, as we see hundreds of medical devices, all these things, and then hundreds of lifestyle things and supplements and drugs and all other wonderful things. It's, yeah, it's no one's ever done this much before that I've seen; it's insane.

And it's such an interesting question of if you, if you remove resources as a constraint, if you remove insurance you need to pay for things as a constraint, and you simply say, "We're going to survey the world of literature, tease out the best science, implement these protocols with the team that we can be highly structured, regimented, and we can then try to slow aging, uh, to a maximum, and then try to rejuvenate, uh, lessen age of the body." If we were to take that to the edges of a scientific, uh, potential, where are we out in the world right now? What are our abilities? And that's what really we're trying to probe is what can science do today? What's the most evidence-based protocol physically possible today?

Yeah, that's what we're doing because we have very few constraints. I mean, I, I do as the end of one participant; I'm basically willing to do anything and everything that the science calls for. And we, yeah, it's like in the year 2022, what can a human imagine is possible with the level of health and wellness that can be achieved with the best scientific evidence and a team of professionals implementing it?

Yeah, and what organ rejuvenation results can be achieved as well? Because, you know, obviously in the future it's going to be possible, but how much can we do today in 2022? A lot of people think when they think of aging, there's this contemplation that in the future there'll be some magic pill that will perform magical functions in the body, and that's maybe true. Like, actually, there's a lot of pills that are magical today, but it really is what we're showing, what we'll tell you about today, is that the way we've gone about doing this is to think about measuring the health of a human body through hundreds of markers of bile fluids and imaging and fitness tests. And then once we have those baselines of measurements, then we proceed forward with rejuvenation with strategies that slow the rate of aging and then regenerate what aging has happened.

Yeah, all quantified as biological age markers as well. As no one else is doing that really, as it's been too hard, as you'll see by the insane extensiveness of, uh, this work over the last 18 months. I think a lot of people would have looked at this, and it, it does, it's so far away from the mean of how most people think about health and wellness and standard of care. I mean, yeah, the standard kind of preventive medicine care is who recommendations for lifestyle and, you know, a couple of screening tests here and there; that's, you know, when you're age 40 plus, typically equals 60 bucks, uh, yeah, it's no comparison. You'll see throughout this video, my body always has evidence of the activities we're engaging.

So you see here, all these red marks; this is from an IPL treatment, if I'm not mistaken, a 515-nanometer, uh, this particular technology was shown to change, uh, genetic expression. And so it's not like a filler or a Botox; you're looking at changing the way—well, yeah, rejuvenating—um, over 1300 genes to the young transcriptomic levels from this therapy, which is, yeah, pretty cool, as, uh, and it seems to be working, again, very nice results, uh, yeah, it's part of one of the many therapies in our skin rejuvenation guideline. So you'll see throughout, I mean, I, I always have, um, a healing face and body from something we've done. This is our first—go ahead.

It's important to note early on that, you know, every test we're doing and every therapy we're doing is we're doing this for the clinical guideline on, uh, you know, exactly how to perform the test to quantify the biological age scientifically accurately or statistically accurately. And the clinical guideline, you know, it's peer-reviewed; it's like, why are we doing this? What is the safety, the inclusion, the exclusion, the evidence base, the, uh, you know, what outcome markers you want to measure, we think it's going to work for, and so on.

Yeah, it, it's that's a really important point, the level, level of rigor and methodical approach we take is unique. It helps prevent errors; it keeps everything tied down, grounded in science, its acquisition; it's pretty crazy. It's like a whole new, uh, medical specialty or a whole new kind of way of doing healthcare in a, in an evidence-based way at n equals one rejuvenation.

That ultrasound machine is the first one we had; we now have a new upgraded system, a new medical hospital-grade. Yes, there's one in the background.

I've got the same one.

Yeah, same one here. We're looking at a whole bunch of, uh, msk markers, so we're looking at tendon ligaments, you know, ankles, knees, shoulder, elbows. And so this was our first baseline. We have a team of sonographers who use our machines, and we are trying to assess like, where was the baseline? I'm chronologically 45; what's the state of affairs of my joints? I don't have, I've never had any surgeries on my joints. How old your ligaments, how old your cartilage, all these things, because all the crew damage of different types of age, you know, cartilage disappears a lot; tendons, they get, uh, you know, inflamed and scarred down and thickened, and that's what we're measuring in different ways with ultrasound and MRI. And it's, it's through a disposition of not accepting that aging and deterioration is inevitable, um, so we want to find state of play, and then once we know that, we want to then search literature or rejuvenation strategies. So this is getting us a baseline and msk of the whole body.

Yep, systematic reviews of the literature for every marker to find, you know, all the latest evidence for, you know, rejuvenating each one of these markers across all the organs and then building that into a clinical guideline which we deploy in a personalized way for Brian.

Yeah, we, we also have a phenomenal team; I think we're up to like 20 or 25 experts from around the world. Oftentimes they'll video in or their local.

Yeah, I like to think of it as like a—well, yeah, we hire a, uh, you know, a local, um, or sometimes not local specialist for each one of your organs, so there's like a different, a different doctor for every organ.

Oh, there we go.

Yeah, that new ultrasound machine. Yeah, this is with the, uh, internal jugular veins, knows this issue wasn't it?

What a wild ride that was. How scary.

Yeah, pretty, pretty unusual as well, the unfortunate.

So there's, uh, we found that I had, you know, these, you have jugular veins here, and they are a matrix transport mechanism to and from the brain, and we found almost accidentally, uh, we were doing measurement protocols, but we found this, and I have congenitally small jugular veins, and it was restricting flow, uh, to and from my brain, which would cause, you know, all kinds of problems in the brain and the lungs and the heart, and it was the first time we found in this protocol something alarming and scary, and we redirected our entire efforts to address the thing for two to three months. It was intense; I thought I may have a stroke, and it was a tough situation.

Yes, from we're doing a routine, you know, whole-body high-accuracy cancer non-contrast MRI, the screening purposes, and, um, we have an enhanced checklist for what, what quantitative markers or qualitative markers to check for, uh, you know, beyond cancer and beyond what normal standard radiologists will report. And it's from that, you know, from that we bring in extra radiology support to review these scans of different specialties for different organs, and they can pick up things normal radiologists can't, and that's how we initially found it on that screening MRI scan, and then we subsequently confirmed it on our more advanced, um, you know, uh, more complex head MRI scan, which looks at the magnetic resonance venogram as well, where you see the veins and the flows of them in the head and neck. We, we found in our research, we looked at, I guess we discussed the issue with two or three experts who specifically have built a career on this particular issue; they perform surgeries. We extensively looked at that as a possibility.

Yeah, but I guess the, the point is we found something potentially alarming, and we resourced it appropriately, and we found solutions to it. We'll get into it a little bit later in the video of how we think, how we solved it.

Yeah, I think we're talking about it more later.

Yeah, some nice, uh, Doppler measurements here. One of the advantages of ultrasounds, you get very nice, uh, Doppler measurements or blood flow measurement speed and type measurements, which you can't really get easily or reproducibly with MRI. It's one of the unique advantages of ultrasound. And like an interesting way to think about this is there's a pyramid of value, and measurements in biofluids are great—blood, saliva—but imaging is uniquely viable. And so you need imaging to quantify biological age in, in most organs. It's very hard to accurately quantify biological age and truly prove rejuvenation of, you know, all, all types of Asian pathology, um, without imaging. And this is, yeah, it's important to notice like research-grade imaging; it's nothing; it's like way beyond what you do in a standard, you go and get an echocardiogram to check your heart kind of thing. It's a very specific protocol with a lot of nuances to make it biostatistically accurate according to our biostatistical biological age market criteria, uh, so yeah, it takes a long time; you have to do a lot of repeat measures for coefficients, different types of coefficients of variation reduction, and yeah, it's a big commitment for someone to do it.

You can see here the MRI protocol; we have it. It took us quite a bit of time to put this together and then get it right, like there were a couple of times that the product of the sequences weren't done correctly; we had to do it again.

Yeah, you gotta train the radiographers how to do it, um, it's complex. That's probably the most extensive, uh, yeah, I mean, for all of these, it's almost the most extensive protocol that's ever been done for each of these organs. I think I've spent maybe 15 to 20 hours in an MRI over the past 12, 18 months.

Yeah, brain age, different brain organ ages, time of age, all the, uh, blood vessel quantification and stuff with the ijv stenosis, whole-body mfk, whole-body msk, whole-body cancer screening, and yeah, you see that in the MRI, you know, you do different types of sequences, and then, you know, beyond what it's done standards, and then you get different types of biological aging markers from each one to see different types of biodegrading processes. So you need all the sequences combined to truly assess, um, as much aging as possible, which no one really does.

Yeah, I think here we're talking about the coil and whether the coil is adequate for the scan we were doing, if I remember that correctly.

Oh, yeah, then they're trying to brass coil here for the thymus, which, uh, which didn't work.

Your hair's looking good here.

So, uh, yeah, I mean, yes, big improvements. Yeah, I mean, I started losing hair in my early 30s; that we, that is, so we'll put that in there; that is true. I do love every second of this. Yes, the Sonic protocol, 3D, Islamic Dixon, a very specific type, specific flip angles and TRS and te's, etc. We're now turning our attention—sorry to step on you—so yeah, yeah, to quantify, uh, final gauge, which no one really tries to rejuvenate, even though it's such an important organ for the whole body. So we turned our attention to thymic rejuvenation; we're just getting started on it, so we don't have anything to report yet.

Yeah, this is the daily morning routine. Yeah, gold, why things, pulse wave velocity, 80 accurate to sphigma core, uh, I think R equals 0.8, not the vascular age, the pulse wave velocity, which they don't show on the screen there, but it's a very nice device, again, pwv measurements, which is a marker of an independent risk predictor and a marker of arterial age, normally in the acrostic femoral, uh, region of the overall arterial organ across the body. And my last pwv measurement, I think was 5.7, is that right?

Yeah, it's age 33 equivalent, which is the maximum you can go because it doesn't start aging until that point onwards. Yeah, I measure blood glucose for a while, and then it's with my routine; it's become so stable, I've just stopped measuring it; it's now at a steady like 82; I think it basically doesn't move, um, it's great. This device, though, really good. Yeah, freestyle library is amazing; very cheap for like the value that you get from it.

That, uh, measurement that we had our screen was all like a year old.

Oh, let's talk, let's talk about this one. Let's go. What's happening here? I think we're actually doing it the wrong way around; it should be like, um, you know, plant, like bottom to top of foot, um, but I think this is the first time we're doing it when we're recording it; we've done it a few times since, and it was fine there, but yeah, this is like, um, uh, sensory nervous system, one of the markers you use for aging, sensory nervous system, because slowly, yeah, the distance you can perceive the two-point discrimination is the technical term, uh, decreases or increases a lot on your feet. So when you're older, you can only tell about five or six centimeters between two points on, on your, your plants outfit, and when you're young, it's about 1.5 centimeters, so massive, massive difference, and it's, you know, partly due to diabetes and peripheral neuropathy and the nerves being most vulnerable, the most distal part, uh, but also other types of aging processes as well.

Difference [Laughter] Sorry, do you see that, uh, there was an American Psycho clip thrown in there? We get that a lot in the comments, don't we? Actually, sorry, are those UV gloves?

Yes.

Yeah.

Oh, nice. I was, um, I was enjoying what you were saying; I was surprised to see the American Psycho. That's fun. I'm not sure if we're doing a great job here; the video is playing, and we're not really talking too much. I guess that's fine.

Yeah, yeah, we spent a lot of time on skin being the largest organ in the body. This is interesting; this, this, uh, these measurements we're doing, like, if you're wondering about your skin age and you get these kinds of measurements, it brings it to life for you; it shows you what the, what the naked eye can't see; it's pretty scary.

Yeah, I mean, this is how I got my kids to wear sunscreen. There's actually a study on that that this type of, uh, you know, UV hyperspectral imaging methodology does increase sunscreen endurance.

I could see why.

Yeah, like that, like a zombie face. Holy, that's another one. We're using autofluorescence for skin measurement, measures yes, as the advanced location and products accumulation in the dermis, mostly specifically, uh, yeah, it just increases from age five of that marker; it's kind of a progressive thing, and yeah, clinically validated as well for in diabetics. So we have here, we're doing, uh, IPL, so we have two lasers, two systems at the clinic; one is IPL and one is a laser system, 532 nanometers and 1064. Yeah, basically for skin rejuvenation, needs a lot of different wavelengths and a lot of different pulse widths, um, and also depends on the individual as well, because, you know, some people can't tolerate something or, you know, light-based therapies that don't tolerate in general. But yeah, it's like you need a lot of different things to, to cover all the markers on skin in a time-efficient manner across, across the whole body, because people just rejuvenate their face, and that's only three percent of body surface of skin surface area, and that's not going to have a systemic effect really. But we, we don't skip anything, uh, even backs of ears and soles of feets and palms of hands and fingernails and toenails and all that stuff. And this particular technology was shown to change genetic expression, so you're not just looking, it's not a, um, like a Botox or a filler; you're actually changing genetic expression with this therapy.

Yeah, you know, massively reverses, uh, collagen loss in the, in the domus as well, which drops about 50 from age 20 to 80 for a type 1 and type 3 and the ratio as well, and, uh, uh, yeah, it's not great for wrinkles, that one laser and microneedling and stuff, but better for that and lots of different topicals, and yeah, LED 638, 830 nanometer High jewels, uh, also good for wrinkles and, uh, yeah, and recovery post, um, IPL or laser or micro needling, etc. This is twice a week, 12 minutes each side. Yeah, it's like cooking your entire body, but without, not the old-school way or they did UV light and actually just increases aging in, you know, you get those, uh, Dima mutations and all your, all your skin cells, which is very bad, skin cancer. But this is, yeah, this is, uh, regenerative and not just for the skin, because the wavelength pantry is quite deep, um, you know, you get msk and, uh, uh, brain, actually, brain benefits as well. Some interesting studies on, uh, brain aging on the transcranial Doppler rcts and, uh, depression, anxiety, uh, traumatic brain injury when that hyperintensities, etc., etc., just from LED light. And we see dramatic changes with the improvements we've made, and so since we started measuring my brain with white matter hyperintensities, the markers went down from age 69 to 48, yeah, in eight months' time.

Yeah, and it was, it was stable before because it was like three or four MRIs that are personalized protocols reduce that. That's a fundamental aging marker that isn't normally considered reversible, um, except that you have like MS, you know, relapsing bidding MS, you know, but then it's more like a, you know, level two kind of autoimmune disease rather than an intrinsic aging, uh, base marker. We also mentioned that the, the pills that we showed a second ago, we stopped doing those because, um, we do so many measurements to see what's working, what's not working; we couldn't find efficacy with the pills, so we discontinued that. We're still doing IPL later. Yeah, um, LED. I was just saying, everyone's like, "Oh, you can't establish causality from multiple—"

Multi-component interventions: N equals one. It's like you can really—like it's—if the effect size is large enough, and you do timings, you know, and you know what the expected um endpoints are at each time point, you can, you know, whether it's working or not with quite high uh causality likelihoods.

And this here, we're looking—we're doing patch testing of various settings of the laser. And uh, we learned why that's important because uh, class, I guess 10 days ago, we increased the—the um—we put more power in on the settings, and I had a reaction—I didn't—interaction—I got burned a little bit. So I have—it's fine, it's not a big deal. It's gonna be—yeah, it's good. No one's ever done it before. This um this uh such an extensive protocol, particularly on the body—it was mostly done on the face—this uh this pulse width uh jewels and uh wavelength combination. But yeah, no one patch tested properly. But I've seen—yeah—and everyone we talked to—no one had a patch test protocol. But we did. We looked at—we did everything. Oh no, yikes! I can't look at this. Yeah, so um, one thing we did is we uh—so I'm on caloric restriction—1977 calories a day or thereabouts—and that's just resulted in me having like around a 6% body fat—since I've lost some fat in my face. And so we go ahead—like—yeah, likewise, I'm 6% as well—same number of calories—like past few years. So we basically—we're—we're trying to figure out what to do with uh facial fat because I mean, really what we're trying to do is—I've got uh two boys—19 and 17—and uh, we're trying to make me biologically identical to them. So that if you were to put me in an MRI or them in an MRI, and you looked at both of our scans, you couldn't tell the difference. To be—yeah—like whole body MRI, whole bit of ultrasound, 100 different devices, skin imaging, eye imaging, hemaging, all the blood tests—can't tell the difference. Yeah, indistinguishable. It's like my—my boys are such great uh sports on this—they let me show you every time we're doing the test—like, "Hey, how do you build up in a ball"—they're always cool to just jump on—like this, for example. There's my son—tap—my 17-year-old's on Talmadge. We've uh used all the imaging techniques on—on his face. And so we were comparing uh facial fat and other measurements in between he and I. And then this is an MRI here—is my 19-year-old—here's me. So you even look at like the—the amount of fat—yeah, 3D MRI, the quantitative volume measurements—because you lose a lot of fat with age, and people think it's like just cosmetic—same with skin—but it's not—that's a very—that's a myth. So yeah, it's essential to have young, healthy fat as an organ—skin is an organ—you need to be young and healthy to uh function correctly and also not to affect all the other organs as well.

So what we did here is we were trying to figure out a solution uh for face fat, but we didn't want to do—see where's it at—oh yeah, here—I can't—ah, let me go back and hold that screen—uh, yeah, I've never had a facial injection—it's uh—so yeah, we did an allograft—um—oh, no anesthetic as well. Yeah, yep. So we injected fat into my face, but we couldn't take my own fat because I don't have any fat. And so the doctor—we spoke to several doctors—uh, said if—if we tried to extract fat from me, we'd probably just create an indentation—there's just nothing to draw. And also it's not truly reduced—like it's like uh, you know, H.A fillers—whatever—they're often not truly rejuvenated. But even if you're taking your own fat, that wouldn't be truly rejuvenative. Whereas this um—yeah—this intervention actually stimulates your net—you know—causes of—presumably the mechanism—really know—you know, lots of different transcription—transcription factor signaling—which activates your natural adipose amusement camel stem cells to grow over the extracellular matrix and actually regrow young, youthful fat. So this is the procedure—whether—sorry, Ellie, go ahead.

Yeah, it's totally different from filler—it's like a truly rejuvenated intervention that can reverse age-related fat loss—it's not like uh a cosmetic thing. So again, we're trying to—the objective of this is—we're trying to—we're trying to rejuvenate—again—to recreate the biological specifications of my 17 or 19-year-old. And so if we can measure my body, we can figure out what the age markers are, and then we go to evidence-based medicine and then try to regenerate those. And so this is—again—we're trying to get like similar bilateral specifications for face as my children. Because the fat organ has a biological age—you know, you accumulate loads of senescent zombie cells in your fat organ—uh, you actually gain fat—you know, in the lower face—it all sags down—and uh—but it's not—it's not just the volume of fat that changes or distribution—it's—it's the quality of the fat as well. So you have to regrow young, healthy fat, and that is like—that's how you rejuvenate the fat organ—the fat is an organ—people always—always forget that—there's different subtypes as well.

Yeah, you're right. We—we didn't—we chose not to use lidocaine because it's a stem cell antagonist. And so uh—yeah—that—that hurts—especially when they put the needle in and they injected the fat. Yeah—should have used Ropivacaine—but we think we forgot. Yeah, we actually got a custom RX of Ropivacaine because we were using repivacaine for the IPL because the laser treatments—the light therapy and laser—can be painful. And so we're looking at topical application of Ropivacaine. We tried it once, and we just uh—we messed up in the application—I think I didn't have it on long enough—we didn't put it on ahead of time. But yeah, we should uh—yeah, people often use lidocaine for msk, PRP, and autologous uh stem cell injections, and it's—yeah—it's—it's a known uh—music—kind of stem cell toxin—and cut—you know—um cartilage cell toxin, bone cell toxin, uh skin stem cell toxin, etc. So yeah, it doesn't seem—it's gonna—I think there's—there's reasonable evidence that it reduces outcomes versus non-cytotoxic anesthetics. Although 3D Recon—there—that's pretty cool. Oh yeah, this is cool. This is the—we did an msk—I guess we need a whole body—yeah—for um muscle age quantification—one of my markers there—or uh, you know, total body muscle percentage—because then you're—you've adjusted for uh body weight, which is—you know—a major flaw that people forget so often. Sarcopenia—and muscle aging—muscle age—muscle body muscle percentage by weights just declines uh with age—I think non-linearly—so uh—which is very, very serious—um—and even calorie restriction can, you know, do that as well. But calorie restrictions shouldn't change the percentage—it just decreases the overall amount—which is why the non-personic based markers for a lot of things when it comes to MRI or volumetrics—they're majorly flawed. So here we have msk on ultrasound and MRI—now looking at—yes—all about combining modalities—because you get disadvantages and advantages of each—looking strong. This is my VO2 max protocol—it was 53.2—so it was—this was on par with uh an elite—at elite athlete that's 18 years old. Oh, hey, Thomas! Hello. This—this is my son Talmadge, he's 17. Hey, let's say hi, Thomas. Hi. College is a good sport because every time we have a new measurement plan, everybody wants to get his data. So we have a biological specification of a 17-year-old, and he's always a good sport to jump in and play with us. He's actually on the blueprint protocol too, so he eats everything I eat. He actually—almost everything. What do you think about that, Talmadge?

I don't know—I'm just so normalized to it that it feels like anything else is weird, so I really don't have any kinds of weapons. Do your friends know what you're doing? Not to the extent—why do you think they would think if they knew what you—how you—what you ate and what your daily regimen was? It probably classifies me in the crazy category. We already know that I go to bed early, and they are—yeah—I've just—developers were vacation—like no one—no one's gonna text me or call me past 8:30 p.m. because it's like they're working on like a math assignment—like I'm just—I'm not an option. So it's kind of nice to have a—yeah—I—I'm trying to be like Talmadge as I go up—when I grow young—yeah—when I grew young. That's right. Oliver and I are going to video of the past 18 months. This is our—our ECG—12-lead ECG—we did this ahead of a therapy we're looking at right now. So this is just a baseline recording, but we are doing—yeah—so like we won't talk about it right now, but we're going to do a therapy actually in the next week uh for my lungs—because in the measurements we did with ultrasound, we found uh some B-lines which were abnormal. We think this is connected to the uh jugular vein stenosis—so small pipes here uh that led—and it had bad posture—which led to white matter hyperintensities—potentially uh aging of the heart—and also potentially problems in the lung—were speculating about that—and we've been working very hard to remedy the damage that was done. So we made a lot of progress reversing white matter hyperintensities from 69 to 48—let me pause—and then we were working hard on the heart rejuvenation—and so now we have this therapy coming up this week—uh—we—we've actually been trying to get this done for the past four months—oftentimes that's what it takes us to do—there's always so many problems with getting these therapies up and going—no one does them—so we have to overcome all these challenges. And so we're excited to see if we can make some meaningful progress on—do you want to go into B-line solver? Well, yeah, it comes right to the point of like uh, you know, everything we're doing is according to a uh experimental clinical practice guideline—it's not just like we're randomly trying stuff—it's—you know—we do a systematic review of the literature, write a clinical practice guideline, get a peer-reviewed—to gold standard guideline criteria—like—agree to—and um—then decide how to clinically act based on that. And you know, hundreds of these guidelines combines um help us like guide on what therapies to try next based on what markers—what biological aging markers—we're seeing across all seven of the organs. And to me, it's been great to see because it is consistent with what I learned in becoming a pilot where you get in the cockpit of an airplane and you have a pre-established checklist of things you do every single time. And so before you fly a plane, you may check a hundred things—that's like walking around the airplane making sure all the gauges are right, the pressures are right, everything's set to go before you take off. And if you, of course, try to keep that in short-term memory, you're guaranteed to miss a few items. And so, as Oliver is saying, the—we maintain an extremely rigorous and methodical structure in all the protocols and the measurements—in the interventions—in the follow-ups—we keep—we're fastidious in the note-taking. And so it really is this structure—protocol—that can scaffold—like Oliver saying—is—is that we uh—I don't remember us really making a mistake that could have been prevented with taking the time—guideline—the protocols. Yeah, every time we go back to conventional medicine as a medical error—it's like pretty much 50% of the time—it's honestly stunning. Yeah, it's uh scary. Yes, the lung—lung age model—you go—you've got very strong lungs—stronger than me as well. Do I really? For the moment—I'm working on it—huh—that would be like the better—actually, you got better—you got better volumes—and um—and like, you know, pfer—but uh—yeah, my—my B-lines are better—so we're kind of even overall—so I need to get down to—what do you have—two B-lines? You're—you're in the normal range? Yeah, yeah, it's about that—yeah—or less—I think you're online currently? Yeah, it's quite a few. Yeah, I'm curious to see if this intervention is going to have any effect there—um—yeah—I mean, I'm hopeful.

Yeah, so this is um—I was—looking—capturing my face—remembering this—so this is one of the very first assessments we did um when we were starting to get our baseline of everything. And the woman here who was coaching me was a phenomenal human—she was so excited about the thing—and she coached me if I was giving birth. Yeah, she was amazing—she made it so much fun—and like, of course, you see this—you've got to put your mouth on the thing, and then you have to uh suck in and blow out like as hard as you can. So it was tremendous pressure, and you're trying to go for your best scores—I forgot how much fun this was—like, what am I doing there? Oh, oh, that's right—I'm pulling—obviously—the myth in spiritual pressure. Okay, so I'm—when I'm sucking in—I think that's uh internal intercostal and diaphragm strength—or external—I was—I always forget—but yeah, that's—that's diaphragm strength—you know, declines massively with age, and no one knows how to measure it accurately—except that's seemingly—so what's amazing about this is you can see how all these measurements start piling up. And so, you know, when we're looking at lungs through ultrasound, we're looking at all the measurements of heart ultrasound, MRI—we have all these fitness tests—so you—you get it—dozens and dozens of markers—you can see here in the lower right—you can see lung age—yep—and so you truly get to characterize—go ahead.

Yeah, you know, massive uh, you know uh—you basically—yeah—you have to use—I have to write my own algorithms and software just to manage all the data because it's just—it's so much—it's uh—it's incomprehensible at scale at the moment, unfortunately. Yeah, so when you have this kind of granularity—a biological measurement—then it—it allows you to systematically work on those—these therapies—and understand whether a given thing is working or not—like—so much—so many of the discussions that people are having in the world—is—it's uh—I guess health and wellness kind of feels like almost a religious debate to me where these—the big camps form—and the carnivores battle the vegans—and the biohackers—you know—yeah—and it's like—I guess I don't really understand uh at that level of abstraction—I guess that makes sense that people talk about that—really the only thing that matters is data—and once you have access to the data, you can abstract out what things are—are not working—and there's not a one-size-fits-all approach—but it really—it enables us to think about this—we are agnostic to what exists in the world—we're just trying to get baseline measurements, measure the efficacy of the interventions against these things, and track them—and really—that's where it would be helpful if the entire field will move that direction—if we could have conversations based on data—and it would—yeah—yeah—you know—it's like a small subset of people that do that—you know—they use—actually measure outcomes of some sort—but you know—there's the normally majorly flawed outcomes—or even if they're not—they're not biostatistically valid in various ways—so it's just a—it's a bit—it's a bit awkward—really. So this here—we're—I took a—I swallowed a pill the size of a baby carrot—and you know—it took 30,000-plus images of my intestinal tract—yeah—pretty gruesome—that one [Laughter]—there it is—yeah—then I took laxatives for six hours—and then it came out 10 hours and 38 minutes later—here we go—easy—yeah—I see—there we go—yeah—be careful—oh, it's beautiful—beautiful—sorry everyone at home who didn't like that kind of stuff—we've done a lot of stuff on hair—I look at the—look at the surf—look there—oh, it's quite—I haven't seen those images actually—which guy is uploaded—yeah—so quantitative hair density assessment—yeah—we did—we also have a volume—we did a lit search and we found all the uh given interventions for a daily application to the scalp—and so we did a custom RX formulation of that—and so keep them chilled in a refrigerator—yeah—the hair rejuvenation guideline—yeah—we—I think we're on like version eight or nine—we're doing uh microneedling here—yeah—not just by itself—but with uh—with topicals as well—otherwise it doesn't work. Oh yeah—yeah—I do as well—0.5 millimeters—Alexis does a much better job than I do on myself—when I do it myself. Oh, there we are—yeah—it's very hard actually. Oh yeah—good old laser diodes—which outperform LEDs by a good margin—so six minutes a day—that's a lot of diodes. Oh, this is the—the blood draw for the—we did hair PRP—so take the blood—spit—centrifugal—to play the rich—ah—I can't look—and then I inject it into the scalp—yeah—that's a painful one—it is—I mean, all these things are painful—this one is particularly—and then we all said our blood tests—always—yeah—it comes back to the—you know—the concept of the rejuvenation athletes—whereas, you know, no pain, no gain—and there is a lot of pain involved—you know—it's like—you know—well, you know subjectively uh and objectively—but you know—it takes discipline to—you—you know—to do all these things—all the lifestyle stuff—get all the tests done—even all the—you know—even all the pharmaceuticals and other—other stuff—you know—take discipline to attend all the doctor's appointments and commit the time to it. So the psychological discipline—which can be like—you know—emotional pain—but also physical pain as well—with like a skin rejuvenation, hair rejuvenation—uh—some tests—you know—which are a bit more invasive—gum injections—all these things—yeah—it takes a—takes courage to—to do this—you're right on the—the pain—I mean—from even the caloric restriction—the 1000 calories—a massive one—yeah—I am always starving—it doesn't matter if I've just eaten—I'm always starving—and so—yeah—and all these treatments—you're right—they—they all have some element of pain to them—a different kind of pain—but yeah—and here we go—the uh—epigenetic age testing using um, you know, Illumina 850k EPIC array—which is gold standard method—because once you have that—that IDAT file—file—that data of the methylation—you can then apply it to all historical and—and future clocks—because some are majorly flawed—you know—it's nice to have a lot of different clocks and all the future ones—because you need to board data for that—because you know—there's a lot of issues with these clocks—you know—they're not really clinically valid—but most of them—and um—we don't really know what it is in a lot of cases—so we'll do a pulp in a minute—is—we uh—to our knowledge—we set a world record last year of epigenetic age reversal—5.1 years in seven months—and uh—like Oliver saying—it kind of needs to be taken with a great assault—because—big barrel assault—yeah—you would say barrel—because—yeah—I mean—yeah—buyers at all these age markers—they have to meet 15 biostatistical uh biological age marking criteria to be uh, you know, fully clinically valid and make sense to use and draw conclusions from—and uh—yeah—epigenetic age is on the way—it's on the way—it's promising—but it's never sufficient by itself—which a lot of people knew—people with no medical background—you know—that—that's what they assume—so I'll measure at the age—you know—that's aging solves—you know—we reverse at the age—but yeah—these people have no medical backgrounds—and they uh—you know—that's looking at it from a very simplistic and biostatistically inaccurate view—unfortunately. Was this PRP? Yeah—preparing the playlist—bathroom—yep—so man—yeah—so the ejections on this—but this one uh hurts—13—first time we did 14 ml injections—and scalp—it felt like my entire head was swollen—and I think it felt like 50 to 100 injections—each one—I don't—I think it was like a ah 7 to 19 gauge needle—it's big—but it hurts—maybe 21—big—ah—I gotta go—I gotta move past this—that's too much—yeah—we're trying to avoid hair transplant aren't we—because like—even if you do hair transplant right—or hair cloning—which is coming out next year in UK—very exciting.

Legit hair cloning? Um, you still have to do all these other things to maintain your hair—uh, you know, density, volume, thickness, and gray and white hair prevention, etc. So yeah, it's going to be done still.

And some of the skin interventions we've done, the skin creams we've had—I was getting blepharitis in both my eyes, and so I will meet the doc to see that. The weekends started using this tea tree oil to clear out the blepharitis, but then I just stopped using these serums, and it went away. But it was an interesting lesson; as we played through the various serums, I've never had blepharitis before in my eyes, so it popped up when using this. But we did a bunch of eye scans: one when we're looking at my IJVs, but then two, is we've done a bunch of eye markers. You can see here, the bottom left, the 3D image. So we've done HQ, quantity, age is very nice, as well as a slit lamp imaging for quantifying the um, different organs of the eye organ system for biological age.

What's a big machine that one? Bigger than mine? You have this machine at your clinic? I got a smaller one.

Yeah, that's a very big one. That's the uh, enhanced depth one, I think. Um, that's pretty cool. Oh no, maybe it's not, maybe something else. Yeah, the eyes are the window to the brain, so you know you can, as markers coming out, um, which I'm developing for, you know, you can see like uh, um, different types of amyloids or protein deposits and uh, like a fusion deposits as well, and quantify them to assess age of both the eye and parts of the brain as well, which is pretty cool.

Oh, same glasses as me. Good choice. Alexis chosen for me. I never wore, uh, worn glasses before.

Yeah, you chose the same model. Uh, well maybe, yeah, maybe I inspired her. I inspired her. But this uh, this test, they often don't do without glasses, and then it's, it's is not accurate for biological aging assessment. Um, you've got to follow the testing guideline. This is for um, it's one of the oldest tests ever made actually for biological age quantification, and literally in 1912 in the paper it's like, show me the age of your eyes, and like, well show me your, show me your result in this test, I can guess your age, and it's really uncanny. It's uh, kind of scary because it's uh, this measures uh, lens stiffness index essentially and ancillary muscle uh, aging as well, but it's how close you can focus your irons. Another one of the markers within the overall eye organ system aging clock right there. That's what we're using for the blepharitis forward.

Yeah, a good old tea tree. I have dry, dry eye condition, so I now do sustain 500 a day. It's really helped. They get right and say, ah, nice job. It's always hard to tell if I'm getting directly in or if it's on that for you.

Oh, you're healthy. Yeah, this was an interesting one. We saw an expert initially; we did an assessment, it was analog and reporting, and we did a second one that was digital. But I have severe hearing loss in my left ear from shooting guns. I grew up, we, we shot a lot of guns, and I'm right dominant, so had a gun down like this way, my left ear is exposed, and so on the frequency ranges were the noise up from the guns, I've got severe damage.

Yeah, I think you're, you know, one, one of your ears was uh, age 61 or so. Yeah, and uh, yeah, gonna need some probably some intro, you know, intra, intra cochlear injections potentially uh, to rejuvenate that and uh, you know, some of that audio, audio cardio is nice as well, but it's annoying to do when you're doing the shooting.

No, we didn't, sadly.

Oh wow. Okay. It's the thing is, we, we um, didn't work sunscreen, ate sugar cereal, and didn't wear headphones. Like it's a kit. Appreciate it.

Yeah, yeah. When we, we find these markers, for example, like we found, you know, the white, my white matter hyperintensity in my brain, we found these markers on my hearing, so it's really motivating when we find something like this, we want to regenerate, rejuvenate it, and so yeah, the ears, like Oliver saying, we're trying to find ways in which we can go about doing this. Look at my crow's feet there. That's this is like a year old, but we've done a pretty good job, and that's part of the, it's probably from uh, caloric restriction and facial fat loss during the, you know, the first half of the protocol, because we're going, you know, level one, two, three, so you're gonna lose that fat and initially as you induce caloric restriction progressively, and then uh, yeah, once you reach the end, the end point, you can start restoring the volume and uh, more aggressively treating the crow's feet with various interventions.

I sat in a sound booth, and we did right various measurements. Yeah, like you know, these uh, hearing, hearing aids, they don't really count as a uh, you know, rejuvenation therapy because they're, they're, you know, they're, they're pretty, they're kind of like a, you know, it's not truly rejuvenating the air, it doesn't regrow the air to the, the era anatomy to uh, younger levels. So a truly rejuvenative therapy would do that, but you know, prosthetics are great, they, they do count in, in some ways, um, but often, yeah, they're currently, they're not, they're not a great solution for um, complete rejuvenation appearing to, you know, age, age 10 levels when the air starts aging. But this is what we typically do here. You're seeing that we, we did the first analog measurement of the hearing, but we, we want a digital because uh, more precise measurement and for our record keeping. So we, we bought a machine. This is typically what happened; we'll take a given area of exploration, we'll find out what's commercially available, and then uh, we'll go see a doctor and do it, and then we'll try to bring up the infrastructure in-house entirely. So we'll buy the equipment ourselves and then hire civil experts, and so our team now is like 20 plus people from around the world, and the clinic we now have, you know, a lot of, a lot of testing equipment. And so building up this expertise in-house is really important.

So here we're doing leads. Yeah, like you know, every test has a guideline as well, and like unless you, if you don't follow the guideline, you can't actually make the assumption that your organ is a certain age because it has to be, you know, if you're using different equipment or different methodologies, then it's going to be totally inaccurate. So you have to use the exact right things to make sure, you know, to enable you to measure the age of an organ. It's very dependent on a lot of variables. You can see here these red marks here on my chin. This is, we found these red spots with the uh, looking at the facial scanner, the skin age markers, and then we use the 532 laser to treat that, and so this is still healing from that. Yeah, and that's it in the background. Good nails, good nail health. You'll see here, oh, let me go back. Yeah, yeah, you're right. Ah, right there. Yeah, we bought this machine, so this is the laser that has 532 and 1064 nanometers. Yeah, square machine that one. I've recently been painting my, my fingernails, which has been fun.

Yeah, it's fun comparing uh, audiograms with uh, with each other. I think is there a picture? Yeah, the loss, there's one of you, you comparing against Talmadge.

Yeah, yeah, so interesting. Yeah, so Talmadge is, we'll show an image in a minute, but he just had hearing that was normal, and then mine crashes down. Yeah, we're testing up to 20,000 Hertz as well, so it's uh, a lot more comprehensive than your standards, you know, one, two, five to eight thousand Hertz.

I'm trying so hard.

Yeah, this one's designed to not require a sound booth. It's uh, clinically validated to, yeah, be done without that sound booth, which is a bigger benefit. These are actually really exhausting. It was 30 to 45 minutes.

Yeah, it takes a while. We did, we did a test two or three times.

Yep. Yeah, there we go. Right there, there's your results. You know, pretty sad story actually. This is our first one; our second one, we had different results, uh, but similar story in terms of the frequency ranges. So we need to figure out if anyone out there knows how to help us rejuvenate my hearing, please get in touch with us. Yeah, I need some uh, advanced therapies for that. It's mostly stem cell and uh, gene therapies that are showing promise in the early studies, which been going on, early clinical studies.

How much rejuvenation have they shown um, in, in mice? It's really good; it's like very big, but uh, uh, I don't think there's been much published data yet for the, or like interim data in the clinical trials yet. So yeah, not sure, still waiting. It's uh, quarter one next year when some of the interim results come out.

I had custom earplugs made. I am sensitive to hearing; I, I don't like loud noises, um, so having some I can just pop in any place. Yeah, active noise-canceling headphones, like they're very good as well, and also uh, Apple Watch, you know, it's got like, um, got the, it's pretty accurate. Yeah, it's got the uh, decibel meter function, which comes on automatically, because you'll be surprised like, you know, even in uh, well especially obviously in shooting, but even in like uh, like a pub or what you think would be a quiet bar, it's still like 90 decibels often, which is, you know, and if you're there for like three hours, it's what my safety levels are 75 decibels. I never go above that.

Yeah, that's very safe. That's very safe. Yeah, 85 is like the standard cutoff, but I like to go for, you know, 80, because it's uh, you know, there's inaccuracy in these decibel meters often as well. You know, that's the thing, like um, as medicine gets better, you can just expose, you can aid your organs, you know, expose yourself to these risks, and then uh, you know, reverse the damage, but we're still, you know, we're on the cusp of that. We can do it in some ways, but um, yeah, the lowest risk strategy, of course, is prevention is better than cure at this point.

So I had uh, bruxism for 20 years, which means I, I would grind my teeth at night, probably due to all the stress of life, and so because of that, I had a lot of recession, and my teeth were in not great shape. So this is one of the very first things we worked on, and we've made uh, remarkable progress. So reduced attachment loss by 41 percent. We did a bunch, a bunch of procedures, and I, we created a daily protocol, so um, yeah, recession's gone down as well, which is, yeah, that's the core aging marker for gums. Yeah, we have a fantastic dentist partner that has worked with us on this, so we, I think we've done six different uh, interventions or six different um, yeah, it turns out injections.

Yeah, yeah, at least the most recent appointment, I, a television, my plaque index was lower than his, and, and my dentist, and your dentist too.

Well, yeah, that's the goal. It's gonna be done. Yeah, CoQ10 for the gums. So that again, like the CoQ10, I'd never heard of this before, and we found this in a study uh, being there was good evidence showing that it had a positive effect on gum health. Yeah, tea tree as well, loads of uh, easy interventions that work on top of flossing and brushing.

Okay, this is a blood draw for the dental procedure. Oh, there's a lot of blood draws. More Manuka honey. That's a lot of Manuka honey. [Laughter] So hard not to eat it.

Yeah, I don't think we have a, a protocol or a Manuka honey application. Manuka honey application, I don't think we measure it. It's just, I was just, just put it on.

Yeah, put on movement, right? Yeah, yeah. We did this minoxidil and uh, PRP with some bone, yeah, some, some good uh, regen meds industry discoveries there, and again, and PRP for gums, gum rejuvenation. Yeah, and most people are might, you know, our dentist partner was saying, no one thinks about rejuvenation in oral health; it's all just a downhill. So she's, she sees somebody, and it's just like, okay, it's gonna get worse. So she was like, everyone was doing our protocol, she would be out of the job, but it's been really energizing for her.

That's a lot. It's a lot. Go ahead.

Oh yeah, there we go. Yeah, that's a beautiful, yeah, my gum age map and the, and the tongue in the middle there on the right. No probing, dab, recession, uh, recession pocket depth, gingivital index, plaque index, calculus index. So pretty comprehensive oral dashboard. It's uh, it's just weird like all hygienists and dentists, well almost all of them, they're just losing, you know, pen and paper on their uh, periodontal reporting forms and uh, and no biological age quantification of course, because no one does that, but that's, you know, the, it's, it's easy to do.

Yeah, there's a lot of green here. There was not as this much green, but we started. Yep, uh, we've had significant success with oral health. Yeah, this first one was like two and a half hours. This procedure was, she doing laser at the end of that as well? She did it. Yeah, the um, I can't remember what wavelength that is, but I think there's a few options. I forget what laser uh, what wavelengths used. Yeah, it's in the guideline, but yeah, also laser, like a lot of, a lot of dentists don't really use laser um, even when it is, it's indicated for a lot more things than what it's normally used for, honestly.

This is an important concept culturally; a lot of people treat sleep as something that can be negotiated on a daily basis. So if the day goes long, or you have a deadline the following day, or whatever, people oftentimes consider sleep to be something that can be squeezed. And I made the decision early on that sleep would be a lighthouse; that it's just a non-negotiable. There's a story behind this; I won't go into it now, but you know, I go to bed at 8:30, and almost nothing can move that time frame. Yeah, sleep regularity index, very important.

Oh yeah, great setup, and my sleep is uh, spectacular. I mean, I've, you know, having three young kids and always being an entrepreneur, my whole life has been like a fair share of stress and turmoil and difficulty, and so sleep has always been something challenging for me, uh, and now it's just um, I love it. It's one of my favorite things I do. Changes how I understand and feel about life. I mean, it changes everything. It changes a lot with age as well, asleep, like you can use it to age the brain in some respects as well, because you know, you're getting like under five hours sleep by age 80 plus, you're getting massive, whasso reduced, the produced um, you know, increase arousals, increase nail hypopnea index, we stop breathing and get sleep apnea, all these things, loads of massive changes in sleep quality and quantity with age, and yeah, you can quantify your sleep age using alert markers as well, and yeah, yours is very good.

Yeah, you know, that's not, again, three and a half hours of REM, two and a half of deep, resting heart rate it was 46, my wake after sleep onset was 24 minutes, which is something we monitor closely. Yep, under 30, normal range. Yeah, it's sort of the trends of these as well, you know, the average for 60 days or, you know, that kind of region. HRV as well, very important. Yeah, we've almost doubled it, yeah, on um, yeah, deep sleep, RMSSD 500, HRV weekly average. This is my morning drink. Do we have the recipe up here? No, we don't. No. Yeah, yeah. I'm so happy. That's pretty, that's pretty. Thank you, Alexis. Um, yeah, it's a good setup because often once we get at this many pills, we have to switch from like the seven-day pill boxes to dedicated morning and evening. Currently 100 pills today, and it, they're all with clinical rationale; it's not just like, oh, let's take 100 pills for no reason. There's like a strict criteria on um, rationale and outcomes for each of these pills and vendor. Yeah, vendor has like quality assurance, GMP, independent testing, etc.

Yeah, maybe at the end of this, it might be worthwhile to pull up the panel of markers just to show where we're at. Oh, this is, yeah, so breakfast is, well, super veggie. What's that? Yeah, yeah, for breakfast every day. Yeah, I love it. So I, you can do it in a non uh, pureed fashion, but I just find this to be, it's more efficient, and it takes quite a while to eat this. This is like uh, I think 300 plus grams of broccoli, 250 grams of cauliflower, garlic, ginger, uh, 250 grams of lentils, black lentils, so it's, it's a lot of volume, and I do that with a tablespoon of olive oil, and then recently I've just added a tablespoon of pure dark chocolate, and that was a surprise. I did not expect dark chocolate to pair well with blended veggies and lentils and olive oil.

You tried that? It's 100, that's why, you know, it's uh, it's cocoa, isn't it? Untouched cocoa rather than having sugar with it, because if it's like actually traditional dark chocolate, which is like 70 or 85, it's gonna be very sugary, and that will taste very weird. But yeah, as it's, as a pure, it's an interesting flavor combination. It's honestly like, I, I drive so much pleasure knowing that I eat this every morning, uh, the density of nutrients from the vegetables, the lentil, olive oil, chocolate uh, as the first thing my body encounters on a daily basis versus a croissant.

This is challenges, so he doesn't do his purees. You can see there's a volume of vegetables, so it's a lot; it takes a long time to eat this. He does chicken. I'm, I'm vegan except for collagen peptides. Yep. Yeah, and it's, it's not like we're saying everyone should do this, you know, with any, any of this stuff, you know, it's all highly personalized, the individual, you know, there's loads of nuances to it, like you've got to build up certain types of food slowly, so you don't get side effects, things. Yeah, this is nutty pudding, so this is uh, macadamia nuts, walnuts, uh, pomegranate juice. Yeah, quarter cup of berries. Yeah, this is basically my, my fat profile. It's delicious. This is the third meal of the day. Let's see. Oh, the fat, third meal of the day was what was it? Yeah, like a salad with like chickpeas, pomegranate seeds, some guacamole. Yep. So uh, in the video we did last year, the blueprint protocol, all three meals were pureed. I think the third meal was the soup, and people are like, hey, wait a second, you're on a geriatric diaper. Yeah, you know, the guy was an 18-year-old, so it's okay.

Yeah, that's one thing the dentist said is my gums are like an 18-year-old; that she, she cannot make my gums bleed. Uh, she says it's amazing that I have a better plaque index than all the teenagers, but I have gum health of a teenager. Yep, it's going to follow the protocol, and yeah, key thing here, you know, there's a lot of uh, meal prep, because prepping this every day, it would be horrible experience, and then yeah, actually, yeah, that's the chocolate. This is 100 pure dark chocolate, which is bitter. Yeah, we have high polyphenol, low heavy metal, so we actually, yeah, I think we should pull that, we have this slide where we showed like, like basically blueprint in five levels of thinking, we'll pull it up to show how we generally think about it, but yeah, just chocolate, it's my favorite. That's also uh, all the extra virgin olive oil we do has six different criteria for the polyphenol density and oleic acid, and so we, we try to be hyper-specific, not just like Oliver saying on the supplements, like everything has a clinical evidence-based rationale, and we then follow the markers, but we did the highest quality inputs everywhere, because there's good evidence that uh, olive oil, extra virgin olive oil that is ultra-premium and meets certain criteria, biochemical criteria, gives better clinical or biomarker outcomes than your regular extra virgin olive oil. That's why we do it, and then you saw there, I have a testosterone patch, that's an Androderm two milligrams patch, which delivers I think uh, nine milligrams of testosterone, something like that.

Yeah, I can never remember this one.

Yeah, yeah, so it's, we, so with the caloric restriction of doing less than 2000 calories, my testosterone dropped to in the four to five hundred range, and so we started supplementing with the Androderm two milligrams patch. Yeah, so I think you had the free testosterone index of age 70 equivalent or so. Yeah, once we've reached, you know, sub 2000 calories with, you know, 2500 is a normal RDA for a man before even doing more for exercise, you know, like lots of exercise, it's a very low caloric intake, and

Yeah, it's kind of like balancing the uh, you know, the benefits of coral restriction and um, and you know, side effects you get. So you can drop testosterone too low. So this is just keeping it in the normal range. It's not, we're not doing like uh, testosterone above the normal range, which is basically not healthy. It's just keeping in the normal range to reduce that biological age of free testosterone index to from 70 to around in the 40s or 30s as well.

Yeah, and to clarify what Oliver was saying, so it's less than 2000 calories, and then I work out rigorously for an hour a day. I have been—I'm actually shocked that I've been able to maintain a healthy body weight and body fat at that level of activity at that caloric count. And so, as Oliver saying, that one of the side effects was lower testosterone, but now we're back up in like eight 800-900 range with that edge, yeah, free testosterone as well.

Yeah, that's right. And this is a non-feminizing estrogen that we worked on. This was—we worked on this for a long time to get this. I could find a supplier and then have our custom uh, Pharmacy compounded for us. This is applied weekly, but one of the most promising interventions we have in terms of lifespan. Yeah, so a lot of therapies, you know, we're using off-label that have been done in uh, that have been shown in Gold standards reproducible Mouse lifespan studies. And yeah, this is one of those examples, and it's been used for decades for hair regrowth over the counter in some countries. And yeah, you know, republishing it longevity, this is the first time I applied this.

Yes, at two minutes in a a small spot. This is my daily workout. This is a year—like a year and a half ago—I was trying to assess where I was at with strange, and so we were doing a baseline single rep mat Max on bench and leg press.

Yep. Yeah, for uh, strength agent, you know, leg age and upper body age muscle age assessments that we've seen this guy. I yeah, again on that East driven, it's not like we're just randomly doing it because the mouse lifespan study did it. There's a whole clinical practice guideline peer-reviewed for every, you know, the rationale behind it, the safety monitoring, the efficacy monitoring, etc. is very, you know, it's a very thought-out process. It's not just like, oh, let's try this on a whim.

Oh, that's a good one. Yeah, that's a lot. Yeah, every day. Some days I wake up and I look—I know this entire list—and I think, but yeah, but I do it every single day, no matter what. And again, you know, this—you've been doing this for a long time now—uh, you know, strength training, not something, you know, we're not saying it's something, you know, there's a guy—there's a guideline for building up strength training. It's not just that you jump straight into this uh, relatively extreme protocol.

Yeah, we're also doing a lot of measurements. We're looking at my whole body measurement of muscle and tendons and ligaments with MRI and ultrasound. So we're able to quantify your dietary intake, fitness routines, and the effect it has across the entire body—tension age, ligament age, joint capsule age, cartilage age, muscle age—across the whole body. So I'm pausing here because uh, the expert we found—when we found my my jugular vein stenosis—a primary problem was I had had bad posture. So you might—I had my chin out and like my neck jug like this, and so I became obsessive about my posture during this process. I still obsess daily about my posture. I probably think about it 40 times a day, more, but always trying to be in a perfect posture, like a imagine a string above my head and pulling me straight up. And so this is one of the PT exercises he has—so elbows straight back and then moving my my shoulders forward, which uh, builds the muscles that I need to keep my neck in the my posture, and this works crazy. It's amazing. It's unbelievable. We had a—with avoided skull-based surgery.

Oh, that was such a scary situation. We talked to two World leading experts that do the surgery, so they go in on the side of the neck uh, which is really complicated because you've got so much happening in your neck with nerves and muscles and and then they shave down the bone, the styloid process uh, to try to eliminate this narrow into the jugular vein. And this expert we had was like, you know, actually like—so we were talking about this—but he's like, you could also make significant progress with this PT. And so we've been working on this now. We saw the study where they've done the surgery, and there was no improvement in clinical outcomes, so it's like, what the hell?

Pretty yeah, pretty worrying. Three people and 22 who received a surgery had sustained benefit. Everyone else and our experts said like, of course, because it's a postural problem fundamentally, like you sure you've got Eagle syndrome and other stuff where people, you know, you've got other structural problems. But interestingly, we did with our new epic 5 ultrasound machine, the the Medical Group hospital-grade machine, we looked at my jugular veins last week, and it's night and day difference. Before, you you could barely see flow in some postural positions, like when I was laying down, but now I'm gonna get it.

Yeah, it was amazing to see the flow uh, how exciting. I mean, especially when I was—I was pretty worried I was gonna have a stroke. Yeah, like morning frontal headaches, you know, raise ICP signs on MRI. Yeah, I'd wake up in the middle of the night with these piercing headaches, and I thought it was COVID, so I'd run downstairs to do a rapid antigen test, and I never had it. I just couldn't understand why I had them. And so when we found the inter—the joke of the vein stenosis—and we started piecing together my symptoms and we sought an MRI and confirmed it was ultrasound.

So yes, I guess I just mentioned this is uh, this to me really is the value of what we're trying to do. We did the extensive measurement in this case. We found something that was potentially life-threatening. We pieced together World experts, again, use multiple measure modalities, implemented protocols, and then measured the progress going forward, and we're able to fix it. And we searched the world over for this thing, and no one knows how to fix this thing systematically, and it was great to see our team succeed so thoroughly. But it can be very serious. Yes, there we go. So yeah, so I'm trying my best to maintain—it's like you have to—yeah, that's really—it's a really hard movement—keep your elbows back and then this is the second one—so trying to strengthen that.

Yeah, let's get that one. And then I put wallpaper—that this makes me so happy to walk in the film—and I feel like I'm in a forest setting. Yeah, I like—they should do that at gyms more often. That's pretty cool. It's good. Yeah, I found a few different prints that had a high enough res it could be wrapped around the whole gym.

Yeah, exactly. It has a significant boost of—yeah—of how I feel every morning. Yeah, yeah, studies on that as well, or even with plants, you know, not great studies, but some personal experience—love a few plants. So this workout takes me—I guess it depends on if I do extra sets on each one—but roughly 43 minutes to complete the whole thing. Yeah, you know, the IJV stuff goes to show, you know, everyone ages differently, not just because of Lifestyle but also, you know, congenital things, which what it might have been in this bilateral idea of the stenosis case. I love this stretch here. Oh, I don't do that one. That's—yeah—looks fun. It helps me feel flexible.

Yeah, flexibility is very good. Yeah, it—I can feel throughout the day if I'm loose or not. This is just to two times doing this in the morning, especially because the offset sitting. Oh yeah, yeah, we both got standing desks, haven't we? And got a cycle chair as well—quite fun—but you really—this workout is designed to take care of basic bodily Health—knees, ankles, hip flexors—your anything on that that forms bad—I should be extending further up—yeah—I'll destroyed because—yeah—flexibility—it's a big part of uh, people don't qualify flexibility, you know, or like introvert or spine—often spine age as well—it's one of the markers for that—and you know, it declines a lot with age. It's really a massive cause of poor quality of life—losing flexibility in poor MSK markers. That was one of the first ones we did uh, one of the first marketers with the the single rep max on bench and then leg press and then we did the stretch instead.

Yep. So I think I was in like the 18 category for all those—AJ team—to all of them above median—like you know, top 10 perhaps. Oh yeah, you know, I didn't really do much hamstring work—going—I guess it was kind of a muscle that was neglected in—yeah—coughs—hamstrings often forgotten about. I'm much better at these now than feet muscles. Yeah, I'm better at these than I I was. This is um, I did—I played a lot of baseball, and so this—this basically works the muscles opposite of throwing a ball. Hey, that's in there twice, is it? No, oh, it's a different one—hands forward, hands back. I do very little biceps and then high-intensity interval training. Yeah, good for uh, cardiac—well, yeah, lots of different organ Rejuvenation actually, but yeah, especially cardiac. If I don't work out on a daily basis, if I don't get this workout in, I just don't feel the same. My body feels a little stiff, right?

Yeah, it's like a—it's like a drug—exercise—the shot feels oddly close up on my chest. That's my son Jefferson. He was—he's an exceptional coach. I love going to the gym and just be like, all right, tell me what to do, and he keeps my form Flawless when otherwise would not be able to do it myself. It's very important to have a coach. Yeah, you actually—you know, Oliver—I don't have a databases—I really miss having Jefferson there on a daily basis. He was so good.

Yep, a lot of evidence for uh, the importance of a coach for, you know, preventing injuries and improving outcomes and you know, longerence to these things as well. Yeah, I'm missing that now because people often just go to the gym and get lost and quit or get injured. Yeah, makes me really miss Jefferson. He's off to college. Yeah, I think you're stronger than Jefferson on a lot of the markers. I am. He—he beats me in several categories um, yeah, the—it's so fun competing with him and Talmadge. We've been—we've actually—so Jefferson was really trying to help me uh, so I could dunk on a on a deck of basketball on a 10-foot rim, and he—I think has succeeded—he's very very close.

Look like you guys are like six foot four. It's quite a challenge. Yeah, we're all six foot fine. Yeah, yeah, we're all identical—same weight, height. Yeah, yeah, maybe more muscle than you. This is—this is Jefferson—the result of Jefferson's coaching. I do really want to perform, so I guess I haven't seen this in a long time—just remembering it. Oh yeah, so this is—oh yeah, that's Talmadge and uh, I'm so happy—that's it. So I think maybe just to wrap this up, all over it may make sense if we just show uh, a few bits from this presentation we have.

Yep. So if I—so one way that that um, Oliver and I've been thinking about this is, you know, we've been—this is just so much fun for us on on for so many reasons, but it's also a project that's kind of hard to communicate because you know, is it—is it just General Health and Wellness? Is it, you know, quote-unquote biohacker? Is it—and how do people discern, you know, does it fall in categories that they understand? Like, is it a vegan diet or a carnivore diet or—and people try to create categories in their mind like, what is this and how do I understand it? And one thing we've been playing with recently is to frame this as uh, what if there was such a thing as a professional Rejuvenation athlete? What would the person's daily lives look like? And that's what we've—we've put in this presentation to share with a few people. And so you can see the Highlight here, like the all the images you just saw in that video—that this is what uh, Oliver was talking about—we're trying to do 78 Oregon biological age measurement—age measurement—and the Rejuvenation, and we rely extensively on scientific back protocols and peer-review Publications. So this is not finding trendy things or uh, debating things in the abstract. It's all based upon science, evidence, and data.

Yeah, and equals one research-grade clinical guidelines peer-reviewed for everything—biased to speed—rigorous uh, biological age measurements—both molecular and phenotypic—across all organs. It's like no one's—no one's coming close to to this. And so if you see like, is it working? How—how are we doing? Here's a dashboard of our latest measurements. Yes, these are some common markers that you know, kind of everyone's somewhat familiar with um, but the key thing here is this isn't just the reference range, which isn't you know, super evidence-based. These are—we go for the uh, you know, like Optimal clinical outcomes range or equal range, which is a you know, a lot more strict than the reference range. And that's the the level where you get the maximum risk reduction in, you know, heart disease, all-cause mortality, good cyber biomarkers, etc. And yeah, we're getting optimal for all of those—which is almost all of those—you needed biological age ring markers in all the organs as well, because all those will still increase with age, but these are still optimal—just slows it down to increase a bit.

That's all. Yeah, exactly. So basically, you think about it in two ways: one is there's slowing the rate of your aging, and then there's reversing the age that has occurred, and we're working on both. So this—yeah—so first is, you know, go ahead. Yeah, get—again—all these optimal—it's just—it's very—it's quite easy relative to actually reversing biological Aging in organs. And yeah, it's not—it's not sufficient at all to um, to rejuvenate organs a large amount, but it's essential because you need a baseline, you need a healthy bodily environment for a lot of the Rejuvenation therapies to stand up at a chance of working as well.

Yeah, so this is again like a list of the measurements we've done, all of which are younger than my chronological age—kind of our trophy dashboard—but it just gives you an idea of examples. So this is not even all—just some—and then we have all these age clocks. So we—we track what we do in terms of the age markers and we'll—how the therapies are working. Yeah, this is a nine months in—quite an early—early dashboard uh, some examples there, but yeah, we're—we're coming up with some organ clocks now, which integrate a lot of different markers inside each clock—so Imaging and devices and biofloads combined. One of the more interesting Concepts uh, Paul here uh, tweeted us out, you know, is generally we are pretty enthusiastic about our technology, and Apple created this culture of excitement around the unveiling of new iterations and new technology, and other companies have followed, and there's—it's cool idea to imagine—could we get into a new place where advances in human Improvement of our health and wellness and Scaffolding up into not just people breaking Olympic records and not—yep—things were accustomed to—but could we really imagine aspiring to human Improvement in ways that were not previously part of our imagination? So can you imagine events happening that are unveiling new ways humans are dramatically improving themselves, and we get ourselves on Improvement curves that are impressive in the same way we look at technology? And so is—is you know, this kind of like—is there a blueprint style keynote that would get people excited about the future of Being Human in a ways in the same way as we look at our technology? Yeah, like we're going from iPhone—iPhone 1 to iPhone 14—but there's no analogy in humans, right? Like everyone that—people haven't even proven they've slowed their aging process a little bit. They're not even measuring their age in a accurate way, and you know, there's no analogy.

It's been fun to share these things publicly. I mean, blueprint is—as you can see—it's difficult to implement in your life because it's pretty extensive in terms of the equipment and the protocols. However, a lot of people have in fact implemented many things from the protocol, and they've seen significant life changes. And so it's been really fun to see people—these are people playing around with the update, you know, looking forward to the updates—but uh, there's—it's been a really great Community that's formed around this, and people are very engaged. They're seeing significant life changes, and I think for a lot of people it has significantly helped them punch through what to do because a common thought process is they'll say, well, yes, first science says this, then they say this—does anybody know what they're talking about? And we've punched through that—we—we don't engage in these high-level abstract arguments of this or that—we just look at the science, the data, and then we, you know, if you look at the dashboard—like, is this working?—yes—like, we've got the data—and we failed this over a long period of time—so we just—there are answers—it's not an unknown area—and you can figure this stuff out because you can have—you know, sure, you need gold standard evidence-based medicine guidelines and everything—but if it's not working for you, it doesn't matter—that's you know, the N equals one data trumps everything.

This is a dashboard of of how this looks like on a daily basis. There's quite a few things we didn't talk about today. We covered a lot of the basics of the Imaging protocols, the exercise, food measurement uh, for example, we didn't talk about senolytics. So these are the zombie cells—when sales stop dividing, they become zombie-like, and they excrete these bad things in the body, and so you want to get rid of them. And senolytics kind of clear them out—like taking out the trash—and so we've implemented—as far as we know—the most advanced symmetic protocol in the world, and we—so we aren't sharing everything—we will share more about this in the future when we have more data. But this—yeah—so much—yeah.

Yeah, we've got a lot more that we have done and are doing which we've not shared, but we will—just to give you something to look forward to. Oh, this is the chocolate thing we were mentioning before, so we really do think about this every time uh, in a methodical way—so clinical guidelines, checklists, yeah, systematic reviews peer-reviewed by a statistical rigorousness on uh, clinically valid biological aging markers. It's the only way it can be done. There's a big difference between if you just think, okay, chocolate is good for you, and then getting to level five and finding the actual untouched test for heavy met—tests for heavy metals from regions of the world—the highest polyphenol density—density uh, there's—that's the difference between this debate of of like we don't know what's good for us and how to pierce through these debates to actually getting results that are repeatable. We do try to take this approach through everything we do.

All right. Yeah, references for everything—not just uh, intuition, which is very dangerous. Oh yeah, the biological age Market criteria—very very important—but quite boring to explain. Yeah, and then we went through all this stuff. This is just the sucky song video of the various things. Yeah, there's some fun images. This is from the laser—we—we chill it very quickly. Oh yeah, this is uh, we mentioned the measurements—this is Talmadge—my 17-year-old—his measurements—as hearing—this is mine. Oh yeah, right—right here—age 61—for update—a thousand minutes left at 51. Yeah, there's a lot of organs I'm not covering in in detail here as well uh, oh yeah, see that in the middle? Yeah, kind of too much to go through. Yeah, we've made—yeah—we made a lot of progress on this. I mean, it—it took a lot to uh, go from zero to where we're at now. We've learned a lot of lessons—we move a lot faster than we ever have uh, but still like we're always pushing the boundaries of what uh, people are familiar with or accept. And so it's—we're constantly pushing through friction. Sexual Health—that you know, this was—a lot of people are familiar with—like basic sexual health uh, panels—but we went pretty extensive on this. It's really something that it should be more robustly measured in life generally um, yeah, the guidelines are quite flawed in

My opinion: the uh, the national ones, yeah. There's a list of the supplements; this is all up on the blueprint website at 148 104 a day fitness. Oh yeah, this is what we mentioned before about epigenetics, and Oliver's mentioned with a barrel of salt, yeah, for lots of reasons.

Yeah, this is the write-up of the jugular vein stenosis, um, which I'm so proud of our team for addressing these as quickly and as thoroughly as we did and achieving the resolution. Yeah, this is a mentioned, so I've got nine b-lines when I should have two, so yeah. And I have gray hair, you know, like I was uh, I think I had gray hair when I was 27 years old, really. I think from my chronic depression, and at that point, not now. And I was, I was an entrepreneur, startup after startup, three young kids, bad relationship, leaving a church I was born into; like, just a really brutal time for me. And we've not yet been able to reverse the gray hair, and it's one of our things we're trying to figure out how to do. Yeah, we've got some strategies, but I think we've uh, yeah, we uh, we prioritize volume first, didn't we? Volume and density before uh, what color? Yeah.

Then we saw this, my my boys being the reference point, you can see the lips in here. Oh yeah, my posture right here, like this is why this was a contributing factor to my my jugular vein being stenosed, is um, shocking to see that. What terrible posture. Yeah, the subclangle is uh, very big, some recent images, so we're trying, I mean, this project is as much health and wellness as it is Artistic for us, that we're just playing around. Yeah, and that's it. All right, any final comments? Any thoughts on your side, Oliver?

Yeah, I was interested to see the um, you know, if we can achieve what people perceive as uh, you know, true scientifically valid multi-organ, you know, organ age rejuvenation results in the next 18 months. I think it's possible; uh, we've done some already. And yes, yeah, I think it's going to be a very exciting result 18 months time. Yeah, that's a great point. We've the majority of our time thus far has been on establishing our measurement infrastructure and getting the computer works. Yeah, diet, exercise, sleep, and so that's really about slowing the rate of aging, yeah, level one step. But recently we've been putting increasing more of our attention on age reversal; we've already started that, several things we haven't talked about, but yeah, I agree with you that the next 18 months are going to be quite exciting. Yeah, it's all about starting with the lowest risk stuff before, you know, increasing the the risk, but also the benefit as well, potential benefits.

So hopefully this was helpful for all of you, even if there's, you know, a small number of things you can implement in your daily life. I mean, I guess if, okay, so if someone's like, all right, give me the TL;DR, what do I do? I mean, what it'd be like a caloric restriction? Yeah. It's tricky. It's tricky because you need a, you need specialist guidance to do that really, uh, and most dietitians aren't trained there. But basically, World Health recommendation, World Health Organization recommendations for the level one stuff, which is all the lifestyle stuff, you know, that recommendations for exercise, science diets, not smoking, 14 units alcohol, BMI in normal range, those are extremely effective, extremely evidence-based and work very well, and only 0.3 of people in the USA actually do all of those to the best extent. So yeah, most of the gains for most people are going to be in the level one stuff, the lifestyle stuff, uh, uh, and yeah, it gets bit more complex after that, even with caloric restriction, because you're going to need clinician oversights, and the clinician needs to be trained in these experimental guidelines, etc.

So lifestyle is never a bad idea; whenever you're in doubt, you go back to WHO recommendations, pretty much as a very simple summary. They're more nuanced in reality, but you know, if you just want a simple solution, it's WHO recommendations, and many of the, I mean, all the protocols we have we share today are on the blueprint website, so the recipes for the daily for food intake for supplements. So if you do engage a doctor or a clinician to help you, you've got a starting point, a reference point on diet, supplements, exercise, and then you can modify it for, you know, your specific circumstances. But hopefully, a point you in the right direction and gives gives you hope that um, you can find answers, that it's not a, it's not an unknowable thing. A lot of people get frustrated thinking that it can't be known, and they're just confused by all different people debating various things, but you can actually achieve these outcomes in a systematic way. You kind of measure the age of your organs, you can try experimental therapies to rejuvenate them in a safe, rational, clinically valid way. That's yeah, that's what we're doing. So we'll post again uh, very soon; we're going to do these more often. And so good luck with uh, whatever you're going to implement; hopefully this will be useful to you over time.